DNA甲基化检测方法及结果准确性分析

王迪; 牛春艳; 王志栋; 杨佳怡; 董莲华; 高运华

doi:10.12338/j.issn.2096-9015.2021.0609

DNA甲基化检测方法及结果准确性分析

doi: 10.12338/j.issn.2096-9015.2021.0609

中国计量科学研究院，北京 100029

基金项目: 中国计量科学研究院基本科研业务费重点领域项目（AKYZZ2025）。

详细信息

作者简介:
王迪（1986-），中国计量科学研究院助理研究员，研究方向：生物计量，邮箱：wangdi@nim.ac.cn

通讯作者:
高运华（1972-），中国计量科学研究院研究员，研究方向：生物计量，邮箱：gaoyh@nim.ac.cn

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出版历程
- 网络出版日期: 2022-03-21
- 刊出日期: 2022-06-02

Detection Methods and Accuracy Analysis of DNA Methylation

National Institute of Metrology, Beijing 100029, China

摘要

摘要: DNA甲基化是一种真核生物中常见的表观遗传学修饰方式，在多种生理及疾病演变过程中发挥了重要作用。甲基化DNA已成为重要的生物标记物，精确定量DNA甲基化水平对临床管理和基础科研都具有重要的价值。但目前基于不同平台和不同方法的检测结果往往有较显著的差异，这种不确定性给它的应用带来了很大的隐患。本文对一些典型的特定位点的甲基化检测方法进行了总结，尤其强调了高灵敏度、高准确性的数字PCR方法在甲基化检测方面的应用，并分析了影响甲基化检测结果准确性的关键因素，最后对建立甲基化定量测量的标准评估体系进行了展望。
- DNA甲基化 /
- 亚硫酸氢盐转换 /
- 生物标志物 /
- 数字PCR /
- 标准物质
Abstract: DNA methylation is a common epigenetic modification in eukaryotes and plays a crucial role in the evolution of many physiological and disease processes. Methylated DNA has become a type of key biomarker, and accurate quantification of the methylation levels is of great value for clinical management and basic scientific research. However, current results based on different platforms and different methods often have more significant differences in results, and this uncertainty poses a great potential for its application. In this paper, we summarize some typical detection methods for the locus-specific methylation analysis, especially highlighting the application of digital PCR in DNA methylation detection with high sensitivity and accuracy, and analyze the key factors affecting the result accuracy, and finally prospect the establishment of a standard evaluation system for quantifying methylated DNA.
- DNA methylation /
- bisulfite conversion /
- biomarker /
- digital PCR /
- reference material

HTML全文

图 1 基于微流控芯片的数字PCR方法检测DNA甲基化示意图^[20]

Figure 1. Schematic diagram of DNA methylation detection by microfluidic chip-based digital PCR assay^[20]

下载: 全尺寸图片幻灯片

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