细胞密度对抗癌药药效评估准确性的研究

薛志超; 赵佳威; 李永淑; 曾嘉明; 龚晓云

doi:10.12338/j.issn.2096-9015.2022.0272

细胞密度对抗癌药药效评估准确性的研究

doi: 10.12338/j.issn.2096-9015.2022.0272

1.
中国计量科学研究院，北京 100029
2.
中国计量大学，杭州 322002
3.
深圳中国计量科学研究院技术创新研究院，深圳 518107

基金项目: 中国计量科学研究院基本科研业务费项目（AKYZZ2224）。

详细信息

作者简介:
薛志超（1988-），中国计量科学研究院博士后，研究方向：细胞计量、蛋白质组研究等，邮箱：xuezc@nim.ac.cn

通讯作者:
龚晓云（1988-），中国计量科学研究院副研究员，研究方向：细胞计量、质谱技术等，邮箱：gxy@nim.ac.cn

中图分类号: TB99
计量
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出版历程
- 收稿日期: 2022-11-16
- 录用日期: 2022-12-08
- 修回日期: 2023-04-07
- 网络出版日期: 2023-04-18

Impact of Cell Density on the Accuracy of Anticancer Drug Efficacy Evaluation

1.
National Institute of Metrology, Beijing 100029, China
2.
China Jiliang University, Hangzhou 322002, China
3.
Shenzhen Institute for Technology Innovation, National Institute of Metrology, Shenzhen 518055, China

摘要

摘要: 使用贴壁细胞进行试验时，细胞接种密度的大小直接关系到细胞贴壁后的密度，是影响后续生物学实验准确性的关键因素。将贴壁Hela细胞系作为研究对象，使用体外实验中常用的3000个细胞/孔和4000个细胞/孔两个密度对细胞进行接种，对比两组细胞接种密度的接种后面积差异、接种后生长速率差异以及对传统化疗药与新型靶向药的药物实验结果差异。结果显示，接种密度为3000个细胞/孔和4000个细胞/孔，两组细胞占每孔底面积有显著统计学差异（P < 0.05）；4000个细胞/孔时细胞生长速率高于3000个细胞/孔。在使用传统化疗药顺铂时，接种密度为3000个细胞/孔及4000个细胞/孔时，第1天、第3天、第5天的药物抑制率无差异，但在使用细胞周期抑制剂帕博西尼时，第1天、第3天、第5天的药物抑制率均有显著差异。因此，细胞的起始接种密度对部分特殊药物的评估存在重要影响。
- 计量学 /
- 细胞计量 /
- 细胞密度 /
- 生长速率 /
- 药物抑制率 /
- 抗癌药物
Abstract: The seeding density of cells, which directly influences the resultant adherent cell density, is a crucial factor affecting the accuracy of subsequent biological experiments. In this study, Hela cell line was used as a model, with two commonly used seeding densities in in vitro experiments: 3000 cells/well and 4000 cells/well. Differences in post-seeding coverage area, growth rates, and the outcomes of drug experiments with traditional chemotherapy and novel targeted therapeutics were compared between these two seeding densities. Significant statistical differences were observed in coverage area between the two groups (P < 0.05). Furthermore, the growth rate at 4000 cells/well was found to be higher than at 3000 cells/well. During the use of the conventional chemotherapy drug, Cisplatin, there was no significant difference in drug inhibition rates between the two cell densities at days 1, 3, and 5. However, when the cell cycle inhibitor Palbociclib was used, significant differences in drug inhibition rates were observed on days 1, 3, and 5. Therefore, the initial seeding density of cells can significantly impact the evaluation of some specific drugs.
- metrology /
- cell measurement /
- cell density /
- growth rate /
- drug inhibition rate /
- anticancer drugs

HTML全文

图 1 细胞底面积差异3000或4000细胞/孔时显微镜下照片

Figure 1. Microscopic images showing differences in cell confluence at 3000 or 4000 cells/well

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图 2 软件计算细胞占底面积比例

Figure 2. Calculation of cell coverage area percentage using software

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图 3 细胞生长差异

Figure 3. Difference in cell growth

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图 4 细胞密度差异对不同药物药效的影响

Figure 4. Impact of cell density differences on the efficacy evaluation of various drugs

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图 5 细胞密度对化疗药药效的影响

Figure 5. Impact of cell density on the efficacy of chemotherapy drugs

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图 6 细胞密度对靶向药药效的影响

Figure 6. Impact of cell density on the efficacy of targeted drugs

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