金属有机框架材料在肿瘤标志物筛查中的研究进展

路欣; 胡亚琳; 屈子裕; 沈丽悦; 董嘉晖; 谢洁; 彭涛

doi:10.12338/j.issn.2096-9015.2022.0287

金属有机框架材料在肿瘤标志物筛查中的研究进展

doi: 10.12338/j.issn.2096-9015.2022.0287

路欣^1,,
胡亚琳^{1, 2},
屈子裕¹,
沈丽悦^{1, 2},
董嘉晖^{1, 2},
谢洁¹,
彭涛^1, ,

1.
中国计量科学研究院，北京 100029
2.
中国计量大学，杭州 310018

基金项目: 国家重点研发计划项目(2022YFF0608402)；中央公益类科研机构基础研究经费(AKYZD2111-2)。

详细信息

作者简介:
路欣（1999-），中国计量科学研究院在读研究生，研究方向：基于纳米材料的免疫快速检测技术，邮箱：13331218159@163.com

通讯作者:
彭涛（1990-），中国计量科学研究院副研究员，研究方向：免疫检测技术与生物计量，邮箱：pengtao@nim.ac.cn

中图分类号: TB99
计量
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出版历程
- 收稿日期: 2022-11-25
- 录用日期: 2022-12-26
- 修回日期: 2022-12-23
- 网络出版日期: 2023-07-07

Research Progress of Metal Organic Frameworks in Tumor Marker

LU Xin^1
,,
HU Yalin^{1, 2},
QU Ziyu¹,
SHEN Liyue^{1, 2},
DONG Jiahui^{1, 2},
XIE Jie¹,
PENG Tao^{1
, ,}

1.
National Institute of Metrology, Beijing 100029, China
2.
China Jiliang University, Hangzhou 310018, China

摘要

摘要: 癌症隐匿性强、发展速度快的特点，给患者疾病治疗造成极大的影响。检测癌症发生与发展中产生的肿瘤标志物对癌症早期诊断、改善预后及降低癌症死亡率具有重大的临床意义。但是，肿瘤标志物的特异度和敏感度不足，单一的肿瘤标志物检测导致误诊或漏诊的可能性增加，也无法提供对特定肿瘤类型的鉴别诊断；肿瘤标志物的检测方法较为复杂，耗时长、成本高，限制了其在临床中的应用。此外，通常人体中肿瘤标志物的含量较低、基质成分复杂，对检测技术的灵敏度和准确度提出了挑战。近年来，金属有机框架（MOFs）材料凭借其独特且优异的物理化学性能，在生物传感器的开发与肿瘤标志物的检测中得到广泛应用。简要综述了MOFs材料的结构、命名方法和功能，并对比了每类MOFs材料的固有特性、优缺点，以及在肿瘤标志物筛查中的应用研究进展，梳理总结了目前存在的问题并提出发展建议，以期为肿瘤标志物的高灵敏新型检测方法开发提供参考。
- 计量学 /
- 癌症 /
- 金属有机框架材料 /
- 肿瘤标志物 /
- 生物传感器
Abstract: Cancer's potent concealment and rapid progression significantly impact patient treatment. Detecting tumor markers produced during cancer's development is of paramount clinical importance for early diagnosis, improving prognosis, and reducing cancer mortality. However, tumor markers' specificity and sensitivity are insufficient, and single tumor marker detection increases the likelihood of misdiagnosis or missed diagnosis, hindering differential diagnosis for specific tumor types. The detection methods for tumor markers are complicated, time-consuming, and costly, limiting their clinical application. Additionally, the generally low levels of tumor markers and complex matrix composition in the human body present challenges for the sensitivity and accuracy of detection techniques. In recent years, metal-organic frameworks (MOFs) have been extensively employed in the development of biosensors and tumor marker detection due to their unique and superior physicochemical properties. This study briefly summarizes the structure, naming methods, and functions of MOFs, compares the inherent characteristics, advantages, and disadvantages of each type of MOFs, and reviews the application progress of MOFs in tumor marker screening. The existing problems and development suggestions are summarized in the hope of providing references for the development of novel, high-sensitivity detection methods for tumor markers.
- metrology /
- cancer /
- metal-organic frameworks /
- tumor markers /
- biosensors

HTML全文

图 1 基于Fe-MOFs的适配体传感器检测CEA^[30]

Figure 1. Aptasensor based on Fe-MOFs for CEA detection^[30]

下载: 全尺寸图片幻灯片

图 2 基于Zn-MOFs的电化学免疫传感器检测肺癌标志物NSE^[42]

Figure 2. Electrochemical immunosensor based on Zn-MOFs for NSE detection^[42]

下载: 全尺寸图片幻灯片

图 3 基于Zr-MOFs的电化学免疫传感器检测CEA^[46]

Figure 3. Electrochemical immunosensor based on Zr-MOFs for CEA detection^[46]

下载: 全尺寸图片幻灯片

图 4 基于La(III)-MOF的“三明治”荧光传感器检测MicroRNA-155^[60]

Figure 4. "Sandwich"-type fluorescent sensor based on La(III)-MOF for MicroRNA-155 detection^[60]

下载: 全尺寸图片幻灯片

表 1 不同金属MOFs材料对比

Table 1. Comparison of different metal MOFs materials

类别	优点	缺点	适用检测方法	检测肿瘤标志物种类
Fe-MOFs	表面易于功能化修饰、吸附封装能力强、氧化还原活性	酸碱稳定性差	电化学传感、免疫层析、适配体传感	胚胎性抗原类、糖抗原类、蛋白质类、酶类
Zn-MOFs	比表面积大、富集发光团	水不稳定、电催化性能差	电化学发光传感、光电化学传感	蛋白质类、酶类、糖抗原类
Zr-MOFs	热/化学稳定性、磷酸基团亲和性、信号放大	光催化性能差	适配体传感、电化学传感	胚胎性抗原类、糖抗原类、蛋白质类、酶类
Cu-MOFs	催化/电化学性能、孔道结构	水不稳定、热不稳定	电化学传感	胚胎性抗原类、蛋白质类
Co-MOFs	热/化学稳定性、高负载能力、活性位点多	酸不稳定、电催化性能差	电化学传感	蛋白质类、激素类、酶类
Ln-MOFs	晶体结构、光学性能出色	光致发光效率低、稳定性差	荧光、电化学发光传感	蛋白质类、胚胎性抗原类
CD-MOFs	生物相容性好、安全性高	水不稳定	电化学发光传感	蛋白质类、酶类

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