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Study on the Impact of Cell Viability Detection and Processing on Drug Effect Evaluation
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摘要: 在使用体外培养的细胞进行药物实验时，药物处理会造成细胞死亡，而检测细胞存活率的方法不同，会对实验结果造成影响。以海拉细胞作为实验对象，用不同浓度的顺铂处理细胞，做去除以及不去除旧培养基的处理，比较包含或不包含旧培养基中漂浮细胞情况下检测得到的细胞存活率。实验结果显示，使用CCK8法检测96孔板、24孔板细胞存活率，不去除旧培养基时实验组细胞存活率高于去除旧培养基实验组细胞存活率。在顺铂浓度为0.625 μM时，不去除旧培养基与去除旧培养基实验组之间的细胞存活率有显著差异。在6孔板和100 mm培养皿中，使用细胞计数仪对用药后的细胞培养基中的漂浮细胞进行检测，发现培养基中漂浮的细胞中存在活细胞。结果表明药物处理后的培养基中存在漂浮的活细胞，去除旧培养基会去除这部分活细胞，从而影响细胞药物实验结果的准确性。Abstract: When conducting drug experiments on cells cultured in vitro, drug treatment can cause cell death, and different methods for detecting cell survival rates can have impacts on the experimental results. This experiment takes Hela cells as the experimental object, and treats the cells with different concentrations of cisplatin. The cell survival rates detected with or without floating cells in the old culture medium are compared. The experimental results indicated that using the CCK8 method to detect the cell survival rates of 96 well plates and 24 well plates, the cell survival rates of the experimental group without removing the old culture medium was higher than that of the experimental group removing the old culture medium. At a cisplatin concentration of 0.625 μM, there is a significant difference in cell survival rates between the experimental group with and without removing the old medium. In 6-well plates and 100 mm dishes, a cell counter was used to detect the floating cells in the cell culture medium after application of drugs, and the presence of viable cells in the floating cells in the medium was found. Therefore, the floating living cells in the culture medium will affect the experimental results of cell drug experiment.
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Key words:
- metrology /
- cell cytometry /
- detection method /
- cell viability /
- drug effects /
- in vitro culture
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图 1 不同药物浓度和不同CCK8孵育时间下的药物作用
Figure 1. The drug effect under different drug concentrations and different CCK8 incubation time
图 2 培养基不同处理方式操作示意图
Figure 2. Schematic diagram of the operation of different processing methods of culture medium
图 3 去除/不去除旧培养基检测的细胞存活率
Figure 3. Cell viability assays with or without removal of old media
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