Volume 66 Issue 4
Jun.  2022
Turn off MathJax
Article Contents
WEI Qi, SU Fuhai. The Determination of 6 Amphetamine Drugs in Hair Samples by GC-MS and LC-MS/MS[J]. Metrology Science and Technology, 2022, 66(4): 48-54. doi: 10.12338/j.issn.2096-9015.2021.0611
Citation: WEI Qi, SU Fuhai. The Determination of 6 Amphetamine Drugs in Hair Samples by GC-MS and LC-MS/MS[J]. Metrology Science and Technology, 2022, 66(4): 48-54. doi: 10.12338/j.issn.2096-9015.2021.0611

The Determination of 6 Amphetamine Drugs in Hair Samples by GC-MS and LC-MS/MS

doi: 10.12338/j.issn.2096-9015.2021.0611
  • Accepted Date: 2022-03-31
  • Available Online: 2022-04-12
  • Publish Date: 2022-06-02
  • A method was developed for the analytical determination of six amphetamine drugs in hair samples by GC-MS and LC-MS/MS. The hair samples were prepared by immersing in the drug-dimethyl sulfoxide solution including target analytes. The sample pretreatment process was optimized using the one-way variable method, and the prepared hair samples were quantified using GC-MS and LC-MS/MS methods. The working curve method was used for quantitative analysis with methamphetamine-D5 as the internal standard. The results showed that the optimal pretreatment methods for the six amphetamine drugs in the prepared hair samples were: 0.01 mol·L−1 HCl, liquid-material ratio 100:1 (mL·g−1), extraction temperature 40 ℃, extraction time 50 min. In GC-MS, the linear range of 6 amphetamine drugs was 0.5 ~ 5.0 ng·mg−1, r2>0.999. The detection limits of the method were 0.04~0.08 ng·mg−1, and the recovery rates were greater than 85.46%, RSDs≤3.6% (n=9). In LC-MS/MS, the 6 amphetamine drugs showed a good linear relationship within the concentration range of 0.06~0.50 ng·mg−1, r2>0.999. The detection limits were 0.05~5.0 pg·mg−1and the recovery of appended contrast in the blank sample ranged from 86.50% to 111.9%, RSDs≤4.2% (n=9). The established methods were used for the detection of amphetamine drugs in human hair, with high sensitivity, good accuracy, and strong specificity. These two methods can be used as the certification method of the reference material of the amphetamine drug in human hair, which could be applied for drug abuse testing and laboratory quality control in the forensic field.
  • loading
  • [1]
    SHI Q, SHI Y, PAN Y, et al. Colorimetric and bare eye determination of urinary methylamphetamine based on the use of aptamers and the salt-induced aggregation of unmodified gold nanoparticles[J]. Microchimica Acta, 2014, 182(3-4): 505-511.
    [2]
    PHD J, RUTKOWSKI B. The prevalence of methamphetamine and amphetamine abuse in North America: a review of the indicators, 1992 ~ 2007[J]. Drug and Alcohol Review, 2008, 27(3): 229-235. doi: 10.1080/09595230801919460
    [3]
    NATH R, KUMAR D, LI T, et al. Metallothioneins, oxidative stress and the cardiovascular system[J]. Toxicology, 2000, 155(1): 17-26.
    [4]
    卢建珍, 谢明仁, 管海彦. 甲基苯丙胺的毒性作用与检测方法[J]. 中国公共安全(学术版), 2020(1): 128-132.
    [5]
    Coning E D, Stolsvik G. United Nations Office on Drugs and Crime: “Methamphetamine Continues to Dominate Synthetic Drug Markets”[J]. Global Smart Update, 2018, 20: 1-16.
    [6]
    KWON NH, LEE YR, KIM HS, et al. Hybrid Solid-Phase Extraction for Selective Determination of Methamphetamine and Amphetamine in Dyed Hair by Using Gas Chromatography-Mass Spectrometry[J]. Molecules, 2019, 24(13): 2501. doi: 10.3390/molecules24132501
    [7]
    ANDO E, HAYASHIDA M, NIHIRA M, et al. GC-MS analysis of methamphetamine and amphetamine in hair of Thai drug addicts[J]. Nihon Arukoru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence, 2004, 39(3): 168-179.
    [8]
    沈敏, 姜宴, 向平, 等. 毛发中违禁苯丙胺类的代谢研究[J]. 质谱学报, 2000(1): 7-13. doi: 10.3969/j.issn.1004-2997.2000.01.002
    [9]
    PASCAL K. , MARION V, VINCENT C. Hair analysis for drug detection[J]. Therapeutic drug monitoring, 2006, 28(3): 442-446. doi: 10.1097/01.ftd.0000211811.27558.b5
    [10]
    KHAJURIA H, NAYAK BP, BADIYE A. Toxicological hair analysis: Pre-analytical, analytical and interpretive aspects[J]. Med Sci Law, 2018, 58(3): 137-146. doi: 10.1177/0025802418768305
    [11]
    KAPP RW. Clarke’s Analysis of Drugs and Poisons, 3rd edition Edited by Anthony C. Moffat, M. David Osselton, and Brian Widdop Publisher: Pharmaceutical Press: London[J]. International Journal of Toxicology, 2016, 25(1): 81-82.
    [12]
    CHEONG J, SUH S, KO BJ, et al. Screening method for the detection of methamphetamine in hair using fluorescence polarization immunoassay[J]. J Anal Toxicol, 2013, 37(4): 217-221. doi: 10.1093/jat/bkt011
    [13]
    SEO MJ, SONG SH, KIM S, et al. Characteristics of Korean patients with methamphetamine use disorder based on the quantitative analysis of methamphetamine and amphetamine in hair[J]. Arch Pharm Res, 2020, 43(8): 798-807. doi: 10.1007/s12272-020-01259-6
    [14]
    CHEN S, MA J, WANG X, et al. UPLC–MS/MS simultaneous determination of methamphetamine, amphetamine, morphine, monoacetylmorphine, ketamine, norketamine, MDMA, and MDA in hair[J]. Acta Chromatographica, 2020, 32(2): 145-148. doi: 10.1556/1326.2019.00615
    [15]
    BRCIC KARACONJI I, BRAJENOVIC N. Evaluation of amphetamine-type stimulant abuse through hair analysis: Results from 12 years of work[J]. Arh Hig Rada Toksikol, 2014, 65(2): 225-230. doi: 10.2478/10004-1254-65-2014-2514
    [16]
    苏福海. 《液相色谱-飞行时间质谱联用仪性能测定方法》国家标准解读[J]. 计量科学与技术, 2020(10): 3-5.
    [17]
    KINTZ P. Hair Analysis in Forensic Toxicology: An Updated Review with a Special Focus on Pitfalls[J]. Current Pharmaceutical Design, 2017, 23(36): 5480-5486.
    [18]
    杨洪国, 李重阳, 丁斌. 毒品与毛发的结合及其影响因素[J]. 中国司法鉴定, 2013(1): 46-49. doi: 10.3969/j.issn.1671-2072.2013.01.008
    [19]
    KINTZ P, CIRIMELE V, LUDES B. Pharmacological criteria that can affect the detection of doping agents in hair[J]. Forensic Science International, 2000, 107(1-3): 325-334. doi: 10.1016/S0379-0738(99)00176-0
    [20]
    TESTORF MF, KRONSTRAND R, SVENSSON S, et al. Characterization of [3H] Flunitrazepam Binding to Melanin[J]. Analytical Biochemistry, 2001, 298(2): 259-264. doi: 10.1006/abio.2001.5364
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(7)  / Tables(11)

    Article Metrics

    Article views (267) PDF downloads(210) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return