Uncertainty Evaluation of Different Quantitative Calibration Methods for the Determination of Vitamin B12 in Infant Formula

WANG Xuxin; LI Xiaomin; ZHANG Qinghe

doi:10.12338/j.issn.2096-9015.2022.0089

Volume 66 Issue 9

Sep. 2022

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WANG Xuxin, LI Xiaomin, ZHANG Qinghe. Uncertainty Evaluation of Different Quantitative Calibration Methods for the Determination of Vitamin B12 in Infant Formula[J]. Metrology Science and Technology, 2022, 66(9): 23-27, 46. doi: 10.12338/j.issn.2096-9015.2022.0089

Citation:

WANG Xuxin, LI Xiaomin, ZHANG Qinghe. Uncertainty Evaluation of Different Quantitative Calibration Methods for the Determination of Vitamin B12 in Infant Formula[J]. Metrology Science and Technology, 2022, 66(9): 23-27, 46. doi: 10.12338/j.issn.2096-9015.2022.0089

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Uncertainty Evaluation of Different Quantitative Calibration Methods for the Determination of Vitamin B12 in Infant Formula

doi: 10.12338/j.issn.2096-9015.2022.0089

National Institute of Metrology, Beijing 100029, China

Available Online: 2022-09-06
Publish Date: 2022-09-30

Abstract

Abstract

In this study, take measurement of the vitamin B12 in infant formula as an example, measurement uncertainty was evaluated through four calibration methods, including standard addition method, standard addition-isotope dilution mass spectrometry method, single point isotope dilution mass spectrometry method, and standard calibration curve isotope dilution mass spectrometry method. The uncertainty evaluation of the four quantitative methods was assessed based on the ISO-GUM guidelines and other uncertainty assessment methods. The uncertainties introduced by the method of repeatability and curve fitting were the primary sources of uncertainty. Furthermore, the uncertainties obtained by the standard addition method were significantly more significant than those obtained by the isotope internal standard method. The comparative study of different quantification methods can lead to a more effective selection of quantification methods and improved measurement accuracy.
- uncertainty evaluation,
- isotope internal standard method,
- standard addition method,
- vitamin B12

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References(19)

References

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