Valuation Method for Milk Somatic Cell Count Reference Materials and the Uncertainty Evaluation

LIU Yingying; ZHOU Yuhu; WANG Ziquan

doi:10.12338/j.issn.2096-9015.2024.0018

Article Contents

Article Navigation > Metrology Science and Technology > 2024 > Accepted Manuscript

LIU Yingying, ZHOU Yuhu, WANG Ziquan. Valuation Method for Milk Somatic Cell Count Reference Materials and the Uncertainty Evaluation[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2024.0018

Citation:

LIU Yingying, ZHOU Yuhu, WANG Ziquan. Valuation Method for Milk Somatic Cell Count Reference Materials and the Uncertainty Evaluation[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2024.0018

Citation:

PDF( 613 KB)

Valuation Method for Milk Somatic Cell Count Reference Materials and the Uncertainty Evaluation

doi: 10.12338/j.issn.2096-9015.2024.0018

1.
National Institute of Metrology, Beijing 100029, China
2.
Harold (Beijing) Technology Co., Ltd, Beijing 100083, China

Received Date: 2024-01-17
Accepted Date: 2024-01-29
Rev Recd Date: 2024-02-21

Available Online: 2024-03-20

Abstract

Abstract

A method based on microscopic imaging flow cytometry was established to measure the concentration of milk somatic cells in the standard substance for milk somatic cell count. This method labels milk somatic cells using the fluorescent dye Ethium Bromide, and the single cell multi parameter microscopic images provided by flow cytometry can effectively assist in identifying target cells in flow cytometry analysis, thereby providing the correctness and repeatability of the gate strategy. Through multiple measurements and analysis of milk somatic cell standard substance samples with different concentrations of somatic cells, it has been proven that this method has good indoor and daytime repeatability. A mathematical model for measuring the concentration of milk somatic cells in a standard substance for milk somatic cell count based on microscopic imaging flow cytometry technology was established. The analysis determined that the uncertainty of this measurement method mainly comes from measurement repeatability, sample pretreatment, reagent addition, gate operation, and measurement volume. Comprehensive evaluation, targeting the candidate V-grade (～1.0×10⁶/mL) of the standard substance for milk somatic cell count prepared in the study, the relative uncertainty of the measurement method for milk somatic cell concentration based on microscopic imaging flow cytometry is 4.84%.
- metrology,
- milk somatic cells,
- cell concentration,
- uncertainty,
- microscopic imaging flow cytometry

FullText(HTML)

References(24)

References

[1]	唐永明, 王锡波. 浅谈牛奶体细胞数在奶牛饲养管理中的应用[J]. 草食家畜, 2006(1): 47-48.
[2]	汪悦, 王炳, 苏汉书, 等. 牛奶体细胞生成与乳品质量和安全的关系[J]. 动物营养学报, 2017, 29(7): 2269-2277.
[3]	田树清, 范艳平. 乳体细胞数(SCC)在奶牛养殖业中的应用[J]. 中国奶牛, 2009(7): 46-47.
[4]	刘晶. 牛奶体细胞数在牧场管理中的应用[J]. 中国乳业, 2012(5): 37-37.
[5]	杨佳怡, 牛春艳, 刘瑛颖, 等. 生鲜牛乳体细胞检测与计量校准必要性研究[J]. 生物技术通报, 2020(5): 16-21.
[6]	中国农垦乳业联盟. 中国农垦乳业联盟产品标准生鲜乳: T/SFLA 001—2019[S]. 北京: 中国农垦经贸流通协会, 2019: 11.
[7]	中华人民共和国农业行业标准. 生鲜牛乳中体细胞测量方法: NY/T800-2004[S]. 北京: 中华人民共和国农业部, 2004: 6.
[8]	Patelp M, Bhata, Markx G H, et al. A comparative study of cell death using electrical capacitance measurements and diele etrophoresis[J]. Enzyme Micmb Tech, 2008, 43(7): 523-530. doi: 10.1016/j.enzmictec.2008.09.006
[9]	马保臣, 秦卓明, 李建基, 等. 奶牛隐性乳腺炎病原菌的分离鉴定和体细胞数及酶的相关性研究[J]. 家畜生态学报, 2006, 27(2): 63-67.
[10]	左仪. 几种体细胞定量测定方法的应用[J]. 中国乳品工业, 1995, 23(1): 36-39.
[11]	方海田, 刘慧燕, 德力格尔桑, 等. 牛乳乳汁电导率变化与体细胞数相关关系的研究[J]. 现代食品科技, 2007, 23(4): 86-88.
[12]	胡松华, 蔡渭明, 杜爱芳, 等. 牛乳电导率和体细胞含量及乳腺感染关系的研究[J]. 中国兽医科技, 1993, 23(6): 1l-13.
[13]	张莹蕾, 汪银锋, 李素平. 牛奶体细胞数的研究进展[J]. 河南畜牧兽医(综合版), 2008, 29(12): 9-10.
[14]	Davis B. H. , Roussel M. , Fest T. , et al.Toward a reference method for leukocyte differential counts in blood: Comparison of three flow cytometric candidate methods[J]. Cytometry, Part A: the journal of the International Society for Analytical Cytology, 2012, 81A(11).
[15]	Heung Kyu Lee, Jennifer M. Lund, Balaji Ramanathan, et al.Autophagy-Dependent Viral Recognition by Plasmacytoid Dendritic Cells [J]. Science, 2007, 315(5817): 1398-1401.
[16]	Rees P , Summers H D , Filby A , et al. Imaging flow cytometry[J]. Nature Reviews Methods Primers, 2022, 2(1). DOI: 10.1038/s43586-022-00167-x.
[17]	Cerrato G , Liu P , Martins I , et al. Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry[J]. Methods in Enzymology, 2019, 2: 632.
[18]	User Manual: XPR & XSR Analytical Balances
[19]	倪育才. 实用测量不确定度评定[M]. 北京: 中国计量出版社, 2009.
[20]	孙乃峰. 浅谈DHI测试报告中的体细胞数(scc)[J]. 中国奶牛, 2008(4): 63-64.
[21]	石璞, 许尚忠. 奶牛DHI中的体细胞测定与牧场管理[J]. 中国奶牛, 2007(6): 22-24. doi: 10.3969/j.issn.1004-4264.2007.06.010
[22]	Milk-Enumeration of somatic cells-Part 1: Microscopic method (Reference method): ISO 13366-1: 2008. [S/OL].https://www.iso.org/standard/40259.html
[23]	张文阁, 刘俊杰. 血细胞技术分析用国家标准物质的研制[J]. 中国粉体技术, 2009(16): 59-64.
[24]	BD Multitest™ 6-color TBNK reagent with optional BD Trucount™ tube. [EB/OL]. [2021-04-01].https://www.bdbiosciences.com/cn/reagents/clinical/systems-kits/bd-trucount-tubes/c/782111.