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ZHAO Yujia, FAN Peilei, LI Ran, LIU Ran, LI Jingjing, ZHAO Haibo, LIANG Liang. Research Progress on Nucleic Acids Isothermal Amplification Methods of Methicillin-Resistant Staphylococcus Aureus[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2024.0039
Citation: ZHAO Yujia, FAN Peilei, LI Ran, LIU Ran, LI Jingjing, ZHAO Haibo, LIANG Liang. Research Progress on Nucleic Acids Isothermal Amplification Methods of Methicillin-Resistant Staphylococcus Aureus[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2024.0039

Research Progress on Nucleic Acids Isothermal Amplification Methods of Methicillin-Resistant Staphylococcus Aureus

doi: 10.12338/j.issn.2096-9015.2024.0039
  • Received Date: 2024-06-30
  • Accepted Date: 2024-07-25
  • Rev Recd Date: 2024-09-26
  • Available Online: 2024-10-24
  • Methicillin-resistant Staphylococcus aureus (MRSA) has become an important pathogen of nosocomial and community infections due to its high drug resistance and high mortality rate. PCR-based detection of mecA gene is considered to be the "gold standard" for the detection of MRSA, but this method cannot get rid of the dependence on precision temperature control instruments and is difficult to meet the needs of on-site detection. Different from traditional PCR amplification, nucleic acid isothermal amplification can react at a constant temperature, greatly simplifying the operation process, and is suitable for a variety of application scenarios such as clinical rapid detection, bedside diagnosis in wards, community primary care, and on-site epidemiological investigations. In recent years, nucleic acids isothermal amplification methods, such as loop-mediated isothermal amplification, recombinase polymerase amplification and rolling circle amplification have been developed for the detection of MRSA. This article reviews them and provides a reference for the on-site detection of MRSA.
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