Abstract: The accuracy of the dry biochemistry analyzer's analytical results directly impacts the clinical diagnosis results. The main metrological characteristics and specific calibration methods are determined by analyzing the technical principles of dry biochemistry analyzers, and representative dry biochemistry analyzers are selected for experimental validation. Based on the calibration method, the metrological characteristics of the dry biochemical analyzer were verified, and the verification results met the requirements of the determined metrological characteristics. The calibration specification of the dry biochemical analyzer was formulated to promote the standardization and standardization of the traceability of the dry biochemical analyzer.