Abstract: An automatic calibration system for cylindrical measuring pins based on LabVIEW was developed. A laser micrometer was used to measure the three pins in a non-contact way. A footswitch was used to control the measurement process of the laser micrometer. The measurement data from one group of three pins was packed up in a "cluster", which was convenient for subsequent automatic sorting according to the specifications of the pins. The electronic original record was automatically generated based on the ActiveX technique and the report was generated after just one click by a LIMS system. The actual operation showed that the system can improve detection efficiency, avoid human errors, and reduce workloads. The system can be widely used in various calibration and testing laboratories.