Abstract: A method was developed for the analytical determination of six amphetamine drugs in hair samples by GC-MS and LC-MS/MS. The hair samples were prepared by immersing in the drug-dimethyl sulfoxide solution including target analytes. The sample pretreatment process was optimized using the one-way variable method, and the prepared hair samples were quantified using GC-MS and LC-MS/MS methods. The working curve method was used for quantitative analysis with methamphetamine-D5 as the internal standard. The results showed that the optimal pretreatment methods for the six amphetamine drugs in the prepared hair samples were: 0.01 mol·L⁻¹ HCl, liquid-material ratio 100:1 (mL·g⁻¹), extraction temperature 40 ℃, extraction time 50 min. In GC-MS, the linear range of 6 amphetamine drugs was 0.5 ~ 5.0 ng·mg⁻¹, r²>0.999. The detection limits of the method were 0.04～0.08 ng·mg⁻¹, and the recovery rates were greater than 85.46%, RSDs≤3.6% (n=9). In LC-MS/MS, the 6 amphetamine drugs showed a good linear relationship within the concentration range of 0.06～0.50 ng·mg⁻¹, r²>0.999. The detection limits were 0.05～5.0 pg·mg⁻¹and the recovery of appended contrast in the blank sample ranged from 86.50% to 111.9%, RSDs≤4.2% (n=9). The established methods were used for the detection of amphetamine drugs in human hair, with high sensitivity, good accuracy, and strong specificity. These two methods can be used as the certification method of the reference material of the amphetamine drug in human hair, which could be applied for drug abuse testing and laboratory quality control in the forensic field.