Abstract: A defibrillation analyzer calibration device based on high-speed discrete sampling integral method is introduced. The device directly measures the defibrillation current and voltage at the load end through high-speed sampling, and calculates the actual output energy through integration and summation to realize the metrological traceability. The basic principle, system composition and hardware and software design of the calibration device are described in detail, and the experiments prove that the main technical specifications of the device meet the design requirements.