Abstract: Certified reference materials (CRM) for sulfur content in light oil-GBW(E)110117, which can be used for the calibration of the UV fluorescence sulfur meter and X-ray fluorescence sulfur meter, was developed to solve the problems in the preparation and characterization of the CRM. The CRM is based on isooctane and polysulfide compounds, and was certified by the internationally recognized primary method of isotope dilution inductively coupled plasma mass spectrometry (ID ICP-MS), and the homogeneity and stability of the CRM candidates were evaluated by UV fluorescence. The results showed that the certified value and its expanded uncertainty were 10.58 mg/kg and 4.3% (k=2), respectively.