Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) has become an important pathogen in nosocomial and community infections due to its high drug resistance and high mortality rate. PCR-based detection of the mecA gene is considered the "gold standard" for detecting MRSA; however, this method relies on precision temperature control instruments and is difficult to meet the needs of on-site detection. Unlike traditional PCR amplification, nucleic acid isothermal amplification occurs at a constant temperature, greatly simplifying the operation process and making it suitable for various application scenarios such as clinical rapid detection, bedside diagnosis, community primary care, and on-site epidemiological investigations. In recent years, nucleic acid isothermal amplification methods such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and rolling circle amplification (RCA) have been developed for the detection of MRSA. This article reviews these methods and provides a reference for the on-site detection of MRSA.