Abstract: The accuracy of portable uric acid analyzers directly affects the daily monitoring of uric acid in patients with gout and hyperuricemia. Due to the lack of calibration standards for these analyzers, it is necessary to establish a calibration protocol. Based on the developed calibration device for portable uric acid analyzers, a calibration method is proposed, and technical indicators are determined. The method is verified through calibration cases, and relevant influencing factors are analyzed. According to the calibration method, the verification results of the indication error and repeatability of the portable uric acid analyzer meet the technical requirements. The successfully established calibration method for portable uric acid analyzers can provide a useful reference for the development of the "Calibration Specification for Portable Uric Acid Analyzers" and promote the traceability and standardization of portable uric acid analyzers.