Abstract: With the rapid development of satellite navigation technology, satellite navigation interference simulation sources are widely used in various applications, including unmanned aerial vehicle (UAV) countermeasures and anti-interference equipment testing and evaluation. This paper focuses on the basic functions and main parameters of spoofing satellite navigation interference simulation sources. Referencing relevant standards and specifications, we conduct an in-depth study on the parameter calibration methods for these sources. We propose calibration methods for RF signals, transmission power, spectral purity, error vector magnitude, interference characteristics, and 1PPS timing error. Additionally, we perform experimental verification and analysis on different models of spoofing satellite navigation interference simulation sources. The results demonstrate that the calibration methods for various parameters are highly operable and can effectively provide scientific and rational metrological traceability for spoofing satellite navigation interference simulation sources. This research promotes the application and development of satellite navigation interference simulation sources.