Abstract: The JJF 1972-2022《Calibration Specification of Performance Parameters of Medical Cabin of Negative Pressure Ambulances》provided accordance for the calibration work of performance parameters of medical cabin of negative pressure ambulances. The metrological characteristics、calibration method and instruments using in measurement process was sorted out, attentions in calibration process were discussed, tracer gas method was a authoritative and recommended method to measure air changes of medical cabin, anemoscope method and tracer gas method were compared and executed, the air changes of medical cabin measured by anemoscope method and tracer gas method were 23.5 h⁻¹ and 17.8 h⁻¹, uncertainty evaluation of the air changes of two methods were discussed, which were 0.8 h⁻¹(k=2) and 1.0 h⁻¹(k=2), the applicable scenario and attentions of photometer method or optical particle counter method in leak detection of HEPA/ULPA was summarized, measure method for airflow pattern of medical cabin of negative pressure ambulances was explained, suggestions for the calibration work of performance parameters of medical cabin of ngative pressure ambulances were made. The application prospect of JJF 1972-2022 was forecasted. Advice for the calibration work of performance parameters of medical cabin of negative was provided.