Abstract: The implementation of JJF 1972-2022, “Calibration Specification of Performance Parameters of Medical Cabins in Negative Pressure Ambulances”, provides a foundational guideline for calibrating the performance parameters of medical cabins in negative pressure ambulances. This paper outlines the metrological characteristics, calibration methods, and standard instruments utilized during the measurement process for these performance parameters. Additionally, it discusses critical considerations during calibration procedures. The tracer gas method is identified as the authoritative and recommended approach for measuring the air change rates within medical cabins. A comparative analysis between the anemoscope (wind speed) method and the tracer gas method was conducted to evaluate their respective advantages and disadvantages in measuring the air change rates of the same negative pressure ambulance medical cabin. The anemoscope method measured an air change rate of 23.5 h⁻¹, while the tracer gas method measured 17.8 h⁻¹. The paper also explores methods for uncertainty evaluation of the air change rates, finding uncertainties of 0.8 h⁻¹ (k=2) and 1.0 h⁻¹ (k=2) for the anemoscope and tracer gas methods, respectively. Furthermore, the study summarizes the applicable scenarios and considerations for using photometric and counting methods in leak detection of HEPA/ULPA filters, and elucidates key points in airflow pattern measurement methods. The application prospects of the JJF 1972-2022 calibration specification are also discussed, providing valuable references for the calibration work of performance parameters in medical cabins of negative pressure ambulances.