Abstract: As an important part of the metrological system, the certified reference material (CRM) is an important means of evaluating analytical methods and ensuring the accuracy of measurement and test results. This solution CRM was prepared using dinotefuran purity CRM (GBW06167) as the raw material and chromatographically pure acetonitrile as the solvent, to prepare at a mass concentration of 1.00 mg/mL using the weight-volume method. To guarantee the accuracy of the measurements, the external calibration method of liquid chromatography was employed for quantitative analysis, as well as homogeneity and stability tests, followed by an uncertainty assessment of the measurement results. The results showed that the homogeneity and stability of CRM were good, and all results met the national first-class CRM requirements and this CRM had a relative expanded uncertainty of 1% (k=2). The successful development of the dinotefuran solution CRM GBW08773 not only provides a reliable reference for pesticide residue detection, but also provides a strong support to the work of related research and regulatory agencies.