GBW(E)100674乙腈中展青霉素溶液标准物质的研制

李先江; 国振; 赵光亮; 李秀琴; 张庆合; 李红梅

doi:10.12338/j.issn.2096-9015.2020.9047

GBW(E)100674乙腈中展青霉素溶液标准物质的研制

doi: 10.12338/j.issn.2096-9015.2020.9047

中国计量科学研究院，北京 100029

基金项目: 国家重点研发计划（2016YFF0201106）

详细信息

作者简介:
李先江（1988-），中国计量科学研究院副研究员，研究方向：食品安全，邮箱：lixianjaing@nim.ac.cn

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出版历程
- 网络出版日期: 2021-06-09

Development of Certified Reference Materials of Patulin in Acetonitrile GBW(E)100674

National Institute of Metrology, Beijing 100029, China

摘要

摘要: 本课题研制了乙腈中展青霉素的溶液标准物质1种, 并已成功申报为国家二级标准物质(GBW(E)100674)。该溶液标准物质以展青霉素纯度国家二级标准物质(GBW(E)100673)为原料，采用重量容量法配制。依据JJF 1343-2012《标准物质定值的通用原则及统计学原理》的要求, 本研究采用液相色谱法对乙腈中展青霉素溶液标准物质进行均匀性、短期稳定性和长期稳定性的评价。定值不确定度包含原料纯度、溶液配制、均匀性、短期稳定性和长期稳定性五个方面引入的不确定度。最终，该溶液标准物质GBW(E)100674的定值结果为100 µg/mL，不确定度为2%。本研究研制展青霉素溶液标准物质的量值具有溯源性和准确性，可以支持国家食品安全检测的需要。
- 展青霉素 /
- 标准物质 /
- 均匀性 /
- 稳定性 /
- 不确定度 /
- 乙腈
Abstract: A solution Certified Reference Material (CRM) of patulin was developed and has been officially approved with the code of GBW(E)100674. This CRM was prepared by using the gravimetric-volumetric method with solid materials of the patulin purity CRM GBW(E)100673. According to the general and statistical principles for characterization of reference materials in JJF 1343-2012, homogeneity and stability tests were carried out with liquid chromatography. Uncertainty was evaluated in consideration of solid purity, solution preparation, homogeneity, short-term stability, and long-term stability. The certified value of the patulin solution is 100 µg/mL with an expanded uncertainty of 2% (k=2). The atulin solution CRM with a traceable and accurate value can support food safety tests in China.
- patulin /
- CRMs /
- homogeneity /
- stability /
- uncertainty /
- acetonitrile

HTML全文

图 1 展青霉素溶液标准物质和溶剂空白的色谱图

Figure 1. Chromatogram of the patulin CRM and blank

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图 2 展青霉素溶液标准物质均匀性检验结果（100 μg/ mL）

Figure 2. Homogeneity test result of the patulin CRM (100 μg/mL)

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图 3 展青霉素标准物质的稳定性变化趋势

Figure 3. Short-term stability of the patulin CRM at 40 ℃

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图 4 展青霉素标准物质的稳定性变化趋势

Figure 4. Short-term stability of the patulin CRM at 4 ℃

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图 5 长期稳定性监测结果

Figure 5. Long-term stability of the patulin CRM

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