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去除杂质干扰的定量核磁共振法进展综述

黄挺 王静羽 万康妮

黄挺,王静羽,万康妮. 去除杂质干扰的定量核磁共振法进展综述[J]. 计量科学与技术,2022, 66(6): 26-30 doi: 10.12338/j.issn.2096-9015.2021.0594
引用本文: 黄挺,王静羽,万康妮. 去除杂质干扰的定量核磁共振法进展综述[J]. 计量科学与技术,2022, 66(6): 26-30 doi: 10.12338/j.issn.2096-9015.2021.0594
HUANG Ting, WANG Jingyu, WAN Kangni. Review of Advances in Quantitative Nuclear Magnetic Resonance Methods for Removing Impurity Interference[J]. Metrology Science and Technology, 2022, 66(6): 26-30. doi: 10.12338/j.issn.2096-9015.2021.0594
Citation: HUANG Ting, WANG Jingyu, WAN Kangni. Review of Advances in Quantitative Nuclear Magnetic Resonance Methods for Removing Impurity Interference[J]. Metrology Science and Technology, 2022, 66(6): 26-30. doi: 10.12338/j.issn.2096-9015.2021.0594

去除杂质干扰的定量核磁共振法进展综述

doi: 10.12338/j.issn.2096-9015.2021.0594
基金项目: 中国计量科学研究院基本科研业务费项目(21-AKYZZ2120-21)。
详细信息
    作者简介:

    黄挺(1979-),中国计量科学研究院研究员,研究方向:化学计量、分析化学等,邮箱:huangting@nim.ac.cn

Review of Advances in Quantitative Nuclear Magnetic Resonance Methods for Removing Impurity Interference

  • 摘要: 定量核磁共振(qNMR)是有机化合物纯度定值的潜在基准方法,可以建立绝大部分有机化合物的计量溯源。分析了近年来建立的可以将杂质峰与待测物的定量峰进行有效分离的定量核磁共振法的原理、优点与挑战,包括高效液相色谱(HPLC)与定量核磁共振法(qNMR)结合的方法:双氘代流动相的HPLC-qNMR、单氘代流动相的HPLC-单信号抑制qNMR、HPLC-双信号抑制qNMR、内标回收校正(ISRC)-HPLC-qNMR、内标校正(ISC)-HPLC-qNMR、色谱辅助定量核磁共振的扩展内标法(EIC)、肽杂质校正定量核磁共振法(PICqNMR),二维核磁共振法:定量异核单量子相关核磁共振法(qHSQC)等,以及HPLC-qNMR-HPLC法。这些方法减少了qNMR中潜在的系统误差,提高了结果的准确性,扩大了qNMR作为计量潜在基准方法和常规分析方法的应用范围。
  • 图  1  扩展内标法的示意图

    注:A为标准溶液的色谱峰面积;I为标准溶液的核磁共振峰面积;A'为样品溶液的色谱峰面积;I'为样品溶液的核磁共振峰面积。

    Figure  1.  Scheme of the extended internal standard method

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出版历程
  • 录用日期:  2022-03-31
  • 网络出版日期:  2022-07-13
  • 刊出日期:  2022-07-29

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