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DNA甲基化检测方法及结果准确性分析

王迪 牛春艳 王志栋 杨佳怡 董莲华 高运华

王迪,牛春艳,王志栋,等. DNA甲基化检测方法及结果准确性分析[J]. 计量科学与技术,2022, 66(4): 55-62 doi: 10.12338/j.issn.2096-9015.2021.0609
引用本文: 王迪,牛春艳,王志栋,等. DNA甲基化检测方法及结果准确性分析[J]. 计量科学与技术,2022, 66(4): 55-62 doi: 10.12338/j.issn.2096-9015.2021.0609
WANG Di, NIU Chunyan, WANG Zhidong, YANG Jiayi, DONG Lianhua, GAO Yunhua. Detection Methods and Accuracy Analysis of DNA Methylation[J]. Metrology Science and Technology, 2022, 66(4): 55-62. doi: 10.12338/j.issn.2096-9015.2021.0609
Citation: WANG Di, NIU Chunyan, WANG Zhidong, YANG Jiayi, DONG Lianhua, GAO Yunhua. Detection Methods and Accuracy Analysis of DNA Methylation[J]. Metrology Science and Technology, 2022, 66(4): 55-62. doi: 10.12338/j.issn.2096-9015.2021.0609

DNA甲基化检测方法及结果准确性分析

doi: 10.12338/j.issn.2096-9015.2021.0609
基金项目: 中国计量科学研究院基本科研业务费重点领域项目(AKYZZ2025)。
详细信息
    作者简介:

    王迪(1986-),中国计量科学研究院助理研究员,研究方向:生物计量,邮箱:wangdi@nim.ac.cn

    通讯作者:

    高运华(1972-),中国计量科学研究院研究员,研究方向:生物计量,邮箱:gaoyh@nim.ac.cn

Detection Methods and Accuracy Analysis of DNA Methylation

  • 摘要: DNA甲基化是一种真核生物中常见的表观遗传学修饰方式,在多种生理及疾病演变过程中发挥了重要作用。甲基化DNA已成为重要的生物标记物,精确定量DNA甲基化水平对临床管理和基础科研都具有重要的价值。但目前基于不同平台和不同方法的检测结果往往有较显著的差异,这种不确定性给它的应用带来了很大的隐患。本文对一些典型的特定位点的甲基化检测方法进行了总结,尤其强调了高灵敏度、高准确性的数字PCR方法在甲基化检测方面的应用,并分析了影响甲基化检测结果准确性的关键因素,最后对建立甲基化定量测量的标准评估体系进行了展望。
  • 图  1  基于微流控芯片的数字PCR方法检测DNA甲基化示意图[20]

    Figure  1.  Schematic diagram of DNA methylation detection by microfluidic chip-based digital PCR assay[20]

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  • 网络出版日期:  2022-03-21
  • 刊出日期:  2022-06-02

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