Advancements in the Detection Methods of Food-Borne Pseudomonas Cocovenenans and Its Exotoxins
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摘要: 椰毒假单胞菌是革兰氏阴性菌,多生长于谷类发酵制品、薯类制品、变质银耳和木耳等农产品表面,并可以产生具有毒性作用的米酵菌酸和毒黄素,影响农产品质量安全。该菌所产生的外毒素会作用于人体大脑、肾脏等实质性器官,对机体消化系统和神经系统等造成损伤,致死率较高,个别案例可达100%。简要阐述了椰毒假单胞菌致病机制及其分型方法,系统论述该菌及其外毒素的检测方法研究进展,展望未来的研究方向,以期为椰毒假单胞菌研究提供参考。Abstract: Pseudomonas cocovenenans, a gram-negative bacterium, predominantly grows on the surface of agricultural products like fermented grains, potato products, spoiled Tremella fuciformis (white fungus), and Auricularia auricula (wood ear fungus). This bacterium produces bongkrekic acid and toxoflavin, toxic substances that compromise the quality and safety of agricultural products. Its exotoxins affect vital human organs such as the brain and kidneys, causing significant harm to the digestive and nervous systems, and in some cases, lead to fatal outcomes, occasionally reaching a 100% fatality rate in individual instances. This paper outlines the pathogenic mechanism and molecular typing methods of Pseudomonas cocovenenans and provides a systematic review of the research progress in detection methods for the bacterium and its exotoxins. Furthermore, the paper discusses future research directions, aiming to serve as a reference for ongoing studies on Pseudomonas cocovenenans.
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Key words:
- metrology /
- Pseudomonas cocovenenans /
- bongkrekic acid /
- toxiflavin /
- detection methods /
- food
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表 1 椰毒假单胞菌及其外毒素检测方法对比分析
Table 1. Comparative analysis of detection methods for Pseudomonas Cocovenenans and its exotoxins
检测对象 方法 优点 不足 椰毒假单胞菌 培养基培养法 操作简单、普及度高 耗时长 环介导等温扩增 特异性强 引物设计要求较高 荧光定量PCR 特异性强 依赖标准曲线定量 数字PCR 特异性强 仪器较昂贵 脉冲场凝胶电泳 分辨率高 操作技术要求高 酶联免疫吸附测定 易携带 干扰因素较多 毒黄素 薄层色谱法 操作方便、设备简单、结果易查 需要标准品比色、样品分离效率易受杂质影响 高效液相色谱法 分离效果好、准确性好 仪器较昂贵 色谱-串联质谱法 分离效果好、特异性强 仪器较昂贵 分光光度计法 操作方便、设备简单 准确度易受样品纯度影响 米酵菌酸 高效液相色谱法 分离效果好、准确性好 仪器较昂贵 色谱-串联质谱法 分离效果好、特异性强 仪器较昂贵 -
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