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细胞存活率的检测处理方式对药物效果评估的影响研究

杨璐璐 龚晓云 曾庆黎 张文权 申屠旭萍 薛志超

杨璐璐,龚晓云,曾庆黎,等. 细胞存活率的检测处理方式对药物效果评估的影响研究[J]. 计量科学与技术,2024, 68(4): 60-65, 44 doi: 10.12338/j.issn.2096-9015.2023.0358
引用本文: 杨璐璐,龚晓云,曾庆黎,等. 细胞存活率的检测处理方式对药物效果评估的影响研究[J]. 计量科学与技术,2024, 68(4): 60-65, 44 doi: 10.12338/j.issn.2096-9015.2023.0358
YANG Lulu, GONG Xiaoyun, ZENG Qingli, ZHANG Wenquan, SHENTU Xuping, XUE Zhichao. Study on the Impact of Cell Viability Detection and Processing Methods on Drug Effect Evaluation[J]. Metrology Science and Technology, 2024, 68(4): 60-65, 44. doi: 10.12338/j.issn.2096-9015.2023.0358
Citation: YANG Lulu, GONG Xiaoyun, ZENG Qingli, ZHANG Wenquan, SHENTU Xuping, XUE Zhichao. Study on the Impact of Cell Viability Detection and Processing Methods on Drug Effect Evaluation[J]. Metrology Science and Technology, 2024, 68(4): 60-65, 44. doi: 10.12338/j.issn.2096-9015.2023.0358

细胞存活率的检测处理方式对药物效果评估的影响研究

doi: 10.12338/j.issn.2096-9015.2023.0358
基金项目: 国家重点研发计划项目(2022YFF0705001);国家自然科学基金项目(21927812、22104136)。
详细信息
    作者简介:

    杨璐璐(2000-),中国计量大学在读研究生,研究方向:细胞计量、蛋白质组研究等,邮箱:S22090710062@cjlu.edu.cn

    通讯作者:

    申屠旭萍(1974-),中国计量大学教授,研究方向:生物防治、生物安全,邮箱:stxp@cjlu.edu.cn

    薛志超(1988-),中国计量科学研究院助理研究员,研究方向:细胞计量、蛋白质组研究等,邮箱:xuezc@nim.ac.cn

  • 中图分类号: TB99

Study on the Impact of Cell Viability Detection and Processing Methods on Drug Effect Evaluation

  • 摘要: 在使用体外培养的细胞进行药物实验时,药物处理会造成细胞死亡,而检测细胞存活率的方法不同,会对实验结果造成影响。以海拉细胞作为实验对象,用不同浓度的顺铂处理细胞,做去除以及不去除旧培养基的处理,比较包含或不包含旧培养基中漂浮细胞情况下检测得到的细胞存活率。实验结果显示,使用CCK8法检测96孔板、24孔板细胞存活率,不去除旧培养基时实验组细胞存活率高于去除旧培养基实验组细胞存活率。在顺铂浓度为0.625 μM时,不去除旧培养基与去除旧培养基实验组之间的细胞存活率有显著差异。在6孔板和100 mm培养皿中,使用细胞计数仪对用药后的细胞培养基中的漂浮细胞进行检测,发现培养基中漂浮的细胞中存在活细胞。结果表明药物处理后的培养基中存在漂浮的活细胞,去除旧培养基会去除这部分活细胞,从而影响细胞药物实验结果的准确性。
  • 图  1  不同药物浓度和不同CCK8孵育时间下的药物作用

    Figure  1.  Drug effects under different drug concentrations and CCK-8 incubation time

    图  2  培养基不同处理方式操作示意图

    Figure  2.  Schematic diagram of the operation of different culture medium processing methods

    图  3  去除/不去除旧培养基检测的细胞存活率

    Figure  3.  Cell viability assays with or without the removal of old media

    图  4  加药后培养基中的细胞数量

    Figure  4.  Number of cells in the medium after drug application

    图  5  漂浮细胞中的活细胞占比

    Figure  5.  Proportion of viable cells among floating cells

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出版历程
  • 收稿日期:  2023-12-21
  • 录用日期:  2023-12-25
  • 修回日期:  2023-12-28
  • 网络出版日期:  2024-03-08

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