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脂质精细结构质谱准确测量方法研究进展

程思敏 谭思源 尹欣驰 龚晓云

程思敏,谭思源,尹欣驰,等. 脂质精细结构质谱准确测量方法研究进展[J]. 计量科学与技术,待出版. doi: 10.12338/j.issn.2096-9015.2024.0010
引用本文: 程思敏,谭思源,尹欣驰,等. 脂质精细结构质谱准确测量方法研究进展[J]. 计量科学与技术,待出版. doi: 10.12338/j.issn.2096-9015.2024.0010
CHENG Simin, TAN Siyuan, YIN Xinchi, GONG Xiaoyun. Research Progress in Accurate Mass Spectrometry-Based Measurement Methods for Lipid Fine Structure[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2024.0010
Citation: CHENG Simin, TAN Siyuan, YIN Xinchi, GONG Xiaoyun. Research Progress in Accurate Mass Spectrometry-Based Measurement Methods for Lipid Fine Structure[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2024.0010

脂质精细结构质谱准确测量方法研究进展

doi: 10.12338/j.issn.2096-9015.2024.0010
基金项目: 国家重点研发计划(2022YFF0705001、2023YFF0613603);国家自然科学基金资助项目(21927812、22104136);中国计量科学研究院基本科研业务费(AKYRC2310、AKYRC2302)。
详细信息
    作者简介:

    程思敏(1996-),中国计量科学研究院博士后,研究方向:精细脂质代谢通路分析、单细胞分析等,邮箱:chengsimin@nim.ac.cn

    通讯作者:

    龚晓云(1988-),中国计量科学研究院副研究员,研究方向:质谱离子源研发,邮箱:gxy@nim.ac.cn

  • 中图分类号: TB99

Research Progress in Accurate Mass Spectrometry-Based Measurement Methods for Lipid Fine Structure

  • 摘要: 脂质是细胞内的重要功能生物分子,其数量庞大,种类繁多,且具有结构多样性。近年来,许多学者致力于脂质精细结构的准确测量,以期发现新型脂质分子(群)作为疾病标志物或开展功能研究。脂质标准物质在脂质的定性定量中发挥着重要作用,但由于脂质精细结构的多样性,其制备和定值都不是一件易事。质谱具有强大的化合物结构精准测量能力,在脂质精细结构测量领域备受关注。脂质精细结构质谱分析方法在近年来取得迅速发展,为人们提供了脂质分子研究新视角,加深了人们对脂质结构多样性和脂质代谢网络的认识,也推动了潜在疾病标志物和代谢通路的发现。目前,基于质谱的脂质精细结构测量方法主要采用新型的离子活化裂解和特异性化学衍生两种策略,实现了多种脂质精细结构异构体有效区分和准确测量。对近年来基于上述原理的质谱测量方法进行了归纳及评述,并对未来脂质标准物质的研制进行了展望。
  • 图  1  以磷脂酰胆碱为例说明脂质结构层级

    Figure  1.  Lipid structural hierarchy illustrated using a representative phosphatidylcholine molecule

    图  2  基于OzID的脂质C=C位置测量原理示意图[13]

    Figure  2.  Schematic diagram illustrating the principle of OzID for measuring lipid C=C location

    图  3  脂质C=C位置异构体的HCD谱图和UVPD谱图对比[28]

    Figure  3.  Comparison of HCD and UVPD MS spectra of lipid C=C location isomers

    图  4  PB光化学衍生用于脂质C=C位置异构体准确测量[40]

    Figure  4.  PB photochemical derivatization used for the accurate measurement of lipid C=C location isomers

    图  5  环氧化反应用于脂质 C=C 位置异构体准确测量[50]

    Figure  5.  The epoxidation reaction used for the accurate measurement of lipid C=C location isomers

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  • 收稿日期:  2024-01-09
  • 录用日期:  2024-01-29
  • 修回日期:  2024-05-15
  • 网络出版日期:  2024-05-23

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