多肽化学合成技术进展及多肽计量发展现状

赵悦; 赵海波; 冷小京; 赵晓宁; 王冰玥

doi:10.12338/j.issn.2096-9015.2024.0215

多肽化学合成技术进展及多肽计量发展现状

doi: 10.12338/j.issn.2096-9015.2024.0215

赵悦^{1, 2,},
赵海波^1, ,,
冷小京²,
赵晓宁¹,
王冰玥¹

1.
北京市计量检测科学研究院，北京 100029
2.
中国农业大学食品科学与营养工程学院，北京 100081

基金项目: 国家市场监督管理总局科技计划项目（2022MK002）；北京市博士后工作经费资助项目（2023-zz-067）。

详细信息

作者简介:
赵悦（1990-），北京市计量检测科学研究院助理研究员，研究方向：生物计量，邮箱：zhaoy@bjjl.cn

通讯作者:
赵海波（1974-），北京市计量检测科学研究院正高级工程师，研究方向：计量检测，邮箱：zhaohb@bjjl.cn

计量
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出版历程
- 收稿日期: 2024-06-30
- 录用日期: 2024-07-09
- 修回日期: 2024-07-31
- 网络出版日期: 2024-09-05

Progress in Peptide Chemical Synthesis and its Metrology

1.
Beijing Institute of Metrology, Beijing 100029, China
2.
China Agricultural University, College of Food Science and Nutritional Engineering, Beijing 100081, China

摘要

摘要: 多肽是生命体中一类重要的有机分子，与蛋白质一起共同参与生命活动。其生物活性强，具有多种生物学功能，已广泛应用于新材料、生物医药、食品、化妆品等领域，具有重要的社会和经济价值。近年来，多肽合成技术快速发展，尤其是多肽化学合成法的迅猛发展，大大加快了多肽产品的研发与应用。面对越来越多的多肽产品上市，市场对于多肽类化合物的生物活性分析需求日益增大，同时对多肽产品质量控制标准化也提出了需求。计量作为测量的科学，能够保证测量结果可复现、可溯源，在多肽研发、生产，以及相关产品质量控制中发挥着非常重要的支撑作用。发展多肽/蛋白质生物计量，支持生物测量的有效性和一致性，最终使测量结果可溯源到国际基本单位制（SI），是摆在计量研究者面前紧迫而艰巨的任务。全面介绍了多肽的化学合成研究策略，讨论了这些策略的优缺点及应用价值，并对多肽/蛋白质计量的发展、应用及挑战进行了综述，以期为多肽/蛋白质产品质量控制工作提供借鉴和参考。
- 计量学 /
- 多肽 /
- 液相合成 /
- 固相合成 /
- 标准物质
Abstract: Peptides are an important class of organic molecules in life bodies, and they participate in life activities together with proteins. Peptides, which have strong biological activity and biological functions, have been widely used in new materials, biological medicine, food, cosmetics and other fields. And they have tremendous social and economic value. In recent years, the rapid development of peptide synthesis technology, especially the rapid development of peptide chemical synthesis method, has greatly accelerated the development and application of peptide products. In the face of more and more peptide products on the market, the market demand for biological activity analysis of peptide compounds is increasing. And at the same time, the standardization of quality control of peptide products is also put forward. As a science of measurement, metrology technology can ensure that the measurement results can be reproduced and traced, and plays a critical role in the development and production of peptides and the quality control of related products. Developing peptide/protein metrology, which focuses on the research to achieve the accuracy and consistency of biological measurement and to ensure the measurement results can be traced to the international system of Units (SI), is an urgent and arduous task for metrology workers. This paper comprehensively introduces the chemical synthesis strategies of peptides, along with the advantages and disadvantages of these strategies, and application values. Meanwhile, the development, application and challenges of peptide/protein metrology are also introduced. This article intends to provide a reference for the quality control of peptide/protein products.
- metrology /
- peptides /
- solution-phase synthesis /
- solid-phase synthesis /
- reference materials

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图 1 多肽固相合成策略基本步骤示意图

Figure 1. Schematic representation of the basic steps of peptide solid-phase synthesis strategy

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