Novel Nanomaterials-Based Fluorescent Assay for Rapid Detection of Glutathione

PENG Tao; JIAO Xueshima; ZHENG Pimiao; LIANG Zhanwei; XIONG Jincheng; DAI Xinhua

doi:10.12338/j.issn.2096-9015.2020.9026

Volume 65 Issue 5

Jun. 2021

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Article Navigation > Metrology Science and Technology > 2021 > 65(5): 40-45

PENG Tao, JIAO Xueshima, ZHENG Pimiao, LIANG Zhanwei, XIONG Jincheng, DAI Xinhua. Novel Nanomaterials-Based Fluorescent Assay for Rapid Detection of Glutathione[J]. Metrology Science and Technology, 2021, 65(5): 40-45. doi: 10.12338/j.issn.2096-9015.2020.9026

Citation:

PENG Tao, JIAO Xueshima, ZHENG Pimiao, LIANG Zhanwei, XIONG Jincheng, DAI Xinhua. Novel Nanomaterials-Based Fluorescent Assay for Rapid Detection of Glutathione[J]. Metrology Science and Technology, 2021, 65(5): 40-45. doi: 10.12338/j.issn.2096-9015.2020.9026

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Novel Nanomaterials-Based Fluorescent Assay for Rapid Detection of Glutathione

doi: 10.12338/j.issn.2096-9015.2020.9026

1.
National Institute of Metrology, Beijing 100029, China
2.
China Jiliang University, Hangzhou 310018, China
3.
China Agricultural University, Beijing 100193, China

Available Online: 2021-05-28
Publish Date: 2021-06-24

Abstract

Abstract

The level of glutathione (GSH) in serum has been correlated with the occurrence of various diseases. In this paper, a fluorescence method that can measure GSH levels rapidly and sensitively was successfully established. In this method, manganese dioxide-coated silica nanoparticles (SiO₂@MnO₂) were synthesized simply and rapidly under ultrasonic conditions using silica nanoparticles as carriers and stabilizers, and fluorescent gold nanoclusters (Arg/ATT/AuNCs) with high fluorescence quantum yields were synthesized using arginine and thymine as ligands. The experimental results showed that the strong reduction of GSH could decompose MnO₂ to release Mn(II) to burst the fluorescence of Arg/ATT/AuNCs, so a fluorescence method based on SiO₂@MnO₂ and Arg/ATT/AuNCs was established to measure GSH in serum. Under optimal conditions, the linear range of the method for quantitative measurement of GSH was from 2.5 to 20 nmol/L with a minimum detection limit of 1.23 nmol/L, which was better than similar methods that have been reported. The relative extended uncertainties of the method were 8.6%, 12.4%, and 18.8% (k = 2) for low, medium, and high GSH concentrations, respectively, with accuracies ranging from 87.0% to 105.6%, which meet the requirements of GSH determination and provide a new technique for sensitive and accurate measurement of GSH in serum.
- glutathione,
- manganese dioxide,
- fluorescent gold nanoclusters,
- fluorescence method,
- rapid detection,
- nanomaterials

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References(21)

References

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