Volume 66 Issue 7
Aug.  2022
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LI Ming, ZHANG Wei. Peptide Purity Measurement Technique Based on Organic Elemental Analysis[J]. Metrology Science and Technology, 2022, 66(7): 18-21. doi: 10.12338/j.issn.2096-9015.2021.0558
Citation: LI Ming, ZHANG Wei. Peptide Purity Measurement Technique Based on Organic Elemental Analysis[J]. Metrology Science and Technology, 2022, 66(7): 18-21. doi: 10.12338/j.issn.2096-9015.2021.0558

Peptide Purity Measurement Technique Based on Organic Elemental Analysis

doi: 10.12338/j.issn.2096-9015.2021.0558
  • Available Online: 2022-01-24
  • Publish Date: 2022-08-04
  • Purity measurement of the peptide is the key to building metrological traceability for the measurement value of peptides in clinical chemistry, pharmaceutical chemistry, food chemistry, and other fields. In addition to the mass balance method, nuclear magnetic resonance method, and hydrolysis method, organic elemental analysis is a new technique for measuring the purity of peptides in chemical metrology. However, a detailed introduction to this technique is yet to be reported. In this paper, a technique for peptide purity measurement based on the analysis of nitrogen, sulfur, and other elements has been established. Using oxytocin as a typical sample, the nitrogen and sulfur elements in the sample were analyzed, the content of nitrogen and sulfur elements in the main component was obtained by deducting the content of nitrogen and sulfur elements in the relevant structural impurities, and the purity results of the peptide were obtained by calculation using the information of the number of atoms and molecular weight of nitrogen and sulfur elements in the molecule of the peptide. The relative standard deviation was less than 1% compared with the measurement results of the authoritative mass balance method. The results of the impurity-corrected organic elemental analysis technique established in this paper are accurate and reliable and can be used for peptide purity standard substance determination.
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  • [1]
    JOSEPHS R D, STOPPACHER N, WESTWOOD S, et al. Concept paper on SI value assignment of purity - Model for the classification of peptide/protein purity determinations[J]. Journal of Chemical Metrology, 2017, 11(1): 1-8. doi: 10.25135/jcm.1.17.02.030
    [2]
    JOSEPHS R D, LI M, SONG D, et al. Key comparison study on peptide purity-synthetic human C-peptide[J]. Metrologia, 2017, 54(1A): 08007. doi: 10.1088/0026-1394/54/1A/08007
    [3]
    JOSEPHS R D, LI M, SONG D, et al. Pilot study on peptide purity-synthetic human C-peptide[J]. Metrologia, 2017, 54(1A): 080011.
    [4]
    李明, 李红梅, 李孟婉. C肽的国际比对及C肽纯度标准物质的研制[J]. 计量技术, 2020(9): 112-114.
    [5]
    JOSEPHS R D, LI M, DAIREAUX A, et al. Key comparison study on peptide purity - synthetic oxytocin[J]. Metrologia, 2020, 57(1A): 08014. doi: 10.1088/0026-1394/57/1A/08014
    [6]
    JOSEPHS R D, LI M, DAIREAUX A, et al. Pilot study on peptide purity - synthetic oxytocin[J]. Metrologia, 2020, 57(1A): 08016. doi: 10.1088/0026-1394/57/1A/08016
    [7]
    LI M, JOSEPHS R D, CHOTEAU T, et al. Identification and accurate quantification of structurally-related peptide impurities for synthetic oxytocin by liquid chromatography–high resolution mass spectrometry[J]. Analytical and Bioanalytical Chemistry, 2021, 413(7): 1861-1870. doi: 10.1007/s00216-021-03154-5
    [8]
    WANG S, WU P, LI M, et al. Mass balance method for SI-traceable purity assignment of synthetic oxytocin[J]. Journal of Pharmaceutical and Biomedical Analysis, 2022, 207: 114401. doi: 10.1016/j.jpba.2021.114401
    [9]
    JOSEPHS R D, STOPPACHER N, DAIREAUX A, et al. State-of-the-art and trends for the SI traceable value assignment of the purity of peptides using the model compound angiotensin I[J]. Trends in Analytical Chemistry, 2018, 101: 108-119. doi: 10.1016/j.trac.2017.09.026
    [10]
    ZHANG W, HUANG T, LI H, et al. Purity Measurement of Human C-Peptide by High Performance Liquid Chromatography and Quantitative Nuclear Magnetic Resonance International[J]. Journal of Peptide Research and Therapeutics, 2018, 24: 391-396. doi: 10.1007/s10989-017-9620-6
    [11]
    HUANG T, ZHANG W, LI H, et al. High performance liquid chromatography-Quantitative nuclear magnetic resonance-High performance liquid chromatography for purity measurement of human insulin[J]. Journal of Liquid Chromatography & Related Technologies, 2018, 41(4): 170-179.
    [12]
    BROS P, JOSEPHS R D, STOPPACHER N, et al. Impurity determination for hepcidin by liquid chromatography-high resolution and ion mobility mass spectrometry for the value assignment of candidate primary calibrators[J]. Analytical and Bioanalytical Chemistry, 2017, 409(10): 2559-2567. doi: 10.1007/s00216-017-0202-4
    [13]
    WU P, YE S, LI M, et al. Impurities identification and quantification for arginine vasopressin by liquid chromatography-high resolution mass spectrometry[J]. Rapid Communication in Mass Spectrometry, 2020, 34(12): e8799.
    [14]
    WU P, LI M, KAN Y, et al. Impurities identification and quantification for calcitonin salmon by liquid chromatography-high resolution mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis, 2020, 186: 113271. doi: 10.1016/j.jpba.2020.113271
    [15]
    JOSEPHS R D, MARTOS G, LI M, et al. Establishment of measurement traceability for peptide and protein quantification through rigorous purity assessment – A review[J]. Metrologia, 2019, 56(4): 044006. doi: 10.1088/1681-7575/ab27e5
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