Measurement Comparison of Plasmid DNA Capability by Digital PCR

WANG Xia; NIU Chunyan; LIU Zheng; LU Lin; DONG Lianhua

doi:10.12338/j.issn.2096-9015.2021.0590

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WANG Xia, NIU Chunyan, LIU Zheng, LU Lin, DONG Lianhua. Measurement Comparison of Plasmid DNA Capability by Digital PCR[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2021.0590

Citation:

WANG Xia, NIU Chunyan, LIU Zheng, LU Lin, DONG Lianhua. Measurement Comparison of Plasmid DNA Capability by Digital PCR[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2021.0590

WANG Xia, NIU Chunyan, LIU Zheng, LU Lin, DONG Lianhua. Measurement Comparison of Plasmid DNA Capability by Digital PCR[J]. Metrology Science and Technology. doi: 10.12338/j.issn.2096-9015.2021.0590

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Measurement Comparison of Plasmid DNA Capability by Digital PCR

doi: 10.12338/j.issn.2096-9015.2021.0590

National Institute of Metrology, Beijing 100029, China

Available Online: 2022-04-11

Abstract

Abstract

Digital PCR (dPCR) is an absolute quantitative technique of nucleic acid, which is widely used in transgenic and gene detection. In order to ensure the accuracy and reliability of digital PCR measurement results in China, 16 laboratories were tested using transgenic plasmid DNA samples. The comparison results showed that the difference between the ratio of foreign gene to internal copy number (<25%) to evaluate the measuring ability of the laboratories. The 16 laboratories were all within the acceptable range, and there was no significant difference among different digital PCR platforms. Due to the influence of plasmid conformation, the absolute copy number content of plasmid DNA measured in 7 laboratories was significantly lower than the reference value. It is suggested that when using digital PCR for quantitative measurement of plasmid DNA copy number, it should be confirmed whether the conformation of plasmid has an effect on amplification.
- digital PCR,
- transgene,
- plasmid DNA,
- nucleic acid,
- copy number ratio,
- copy number concentration

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References(21)

References

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