Development and Application of Proficiency Testing Material for Detection of Escherichia Coli in Drinking Water

LI Manli; FU Boqiang; TANG Zhiyu; SHEN Qingfei; ZHANG Yan

doi:10.12338/j.issn.2096-9015.2021.0653

Volume 66 Issue 6

Jul. 2022

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LI Manli, FU Boqiang, TANG Zhiyu, SHEN Qingfei, ZHANG Yan. Development and Application of Proficiency Testing Material for Detection of Escherichia Coli in Drinking Water[J]. Metrology Science and Technology, 2022, 66(6): 60-64. doi: 10.12338/j.issn.2096-9015.2021.0653

Citation:

LI Manli, FU Boqiang, TANG Zhiyu, SHEN Qingfei, ZHANG Yan. Development and Application of Proficiency Testing Material for Detection of Escherichia Coli in Drinking Water[J]. Metrology Science and Technology, 2022, 66(6): 60-64. doi: 10.12338/j.issn.2096-9015.2021.0653

Citation:

LI Manli, FU Boqiang, TANG Zhiyu, SHEN Qingfei, ZHANG Yan. Development and Application of Proficiency Testing Material for Detection of Escherichia Coli in Drinking Water[J]. Metrology Science and Technology, 2022, 66(6): 60-64. doi: 10.12338/j.issn.2096-9015.2021.0653

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Development and Application of Proficiency Testing Material for Detection of Escherichia Coli in Drinking Water

doi: 10.12338/j.issn.2096-9015.2021.0653

National Institute of Metrology, Beijing 100029, China

Accepted Date: 2022-04-06

Available Online: 2022-04-12

Publish Date: 2022-07-29

Abstract

Abstract

The number of microorganisms in domestic drinking water is one of the important monitoring items for water quality safety. To verify the understanding of domestic testing laboratories for the measurement of Escherichia coli (E.coli) in drinking water, the National Institute of Metrology (NIM) developed a proficiency testing sample for the quantification of E.coli in drinking water and carried out a proficiency testing to assess the ability of participating laboratories. Complied with the national standard GB/T 5750.12-2006 Standard examination methods for drinking water - Microbiological parameters, 11 laboratories participated in the proficiency testing and determined the CFU or MPN value of E.coli in lyophilized samples reconstituted in sterile water by using the filtration membrane method and enzyme substrate method. The statistics indicated that the results of all the participating laboratories are satisfactory. The E.coli quantitative proficiency testing samples are homogeneous and stable, which can meet the requirement of proficiency testing for quantification of E.coli in drinking water and reflect the ability of participating laboratories.
- drinking water,
- microorganism,
- Escherichia coli,
- filtration membrane method,
- enzyme substrate method,
- proficiency testing

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References(20)

References

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