Volume 67 Issue 10
Oct.  2023
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GUO Wenbo, MENG Jiajia, NIU Xueke, WANG Xinyi, NIE Dongxia, ZHAO Zhihui, HAN Zheng. Development of a Certified Reference Material for Fumonisin B1 Purity[J]. Metrology Science and Technology, 2023, 67(10): 46-53. doi: 10.12338/j.issn.2096-9015.2023.0284
Citation: GUO Wenbo, MENG Jiajia, NIU Xueke, WANG Xinyi, NIE Dongxia, ZHAO Zhihui, HAN Zheng. Development of a Certified Reference Material for Fumonisin B1 Purity[J]. Metrology Science and Technology, 2023, 67(10): 46-53. doi: 10.12338/j.issn.2096-9015.2023.0284

Development of a Certified Reference Material for Fumonisin B1 Purity

doi: 10.12338/j.issn.2096-9015.2023.0284
  • Received Date: 2023-11-17
  • Accepted Date: 2023-11-20
  • Rev Recd Date: 2023-11-29
  • Available Online: 2023-12-06
  • Publish Date: 2023-10-18
  • To enhance the accuracy, reliability, and traceability of agricultural product quality safety testing, a certified reference material (CRM) for fumonisin B1 (FB1) purity was developed. The CRM candidate, obtained through laboratory purification of FB1, was qualitatively identified using mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The purity of the candidate was quantified using both mass balance and quantitative nuclear magnetic resonance methods. The mass balance method included area normalization, the Karl Fischer method, gas chromatography-tandem mass spectrometry, and inductively coupled plasma mass spectrometry to determine the main component, moisture, volatile and non-volatile impurity contents. Furthermore, homogeneity tests and stability studies were conducted, along with systematic uncertainty evaluations. The results indicated that the FB1 CRM has a purity value of 99.1% with an extended uncertainty of 0.3% (k=2), exhibiting good homogeneity and fulfilling a 12-month stability requirement. The developed FB1 CRM, certified as a national secondary standard material (GBW (E) 100550), is suitable for qualitative and quantitative detection, method evaluation, and establishing a traceability system for FB1 in agricultural products
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  • [1]
    KAMLE M, MAHATO D K, DEVI S, et al. Fumonisins: Impact on agriculture, food, and human health and their management strategies[J]. Toxins, 2019, 11(6): 328. doi: 10.3390/toxins11060328
    郭志青, 张霞, 刁立功, 等. 镰刀菌及其伏马毒素的危害和防控[J]. 山东农业科学, 2022, 54(1): 157-164.
    PONCE-GARCíA N, SERNA-SALDIVAR S O, GARCIA-LARA S. Fumonisins and their analogues in contaminated corn and its processed foods – a review [J]. Food Additives & Contaminants: Part A, 2018: 2183-2203.
    CHEN J, WEI Z, WANG Y, et al. Fumonisin B1 : mechanisms of toxicity and biological detoxification progress in animals [J]. Food and Chemical Toxicology, 2021, 149(3): 111977.
    DEEPTHI B V, SOMASHEKARAIAH R, POORNACHANDRA R K, et al. Lactobacillus plantarum MYS6 ameliorates fumonisin B1-induced hepatorenal damage in broilers[J]. Frontiers in Microbiology, 2017, 8: 2317. doi: 10.3389/fmicb.2017.02317
    SORIANO J M, GONZáLEZ L, CATALá A I. Mechanism of action of sphingolipids and their metabolites in the toxicity of fumonisin B1[J]. Progress in Lipid Research, 2005, 44(6): 345-56. doi: 10.1016/j.plipres.2005.09.001
    OSTRY V, MALIR F, TOMAN J, et al. Mycotoxins as human carcinogens-the IARC Monographs classification[J]. Mycotoxin research, 2017, 33: 65-73. doi: 10.1007/s12550-016-0265-7
    U. S. Food and Drug Administration. guidance for industry: fumonisin levels in human foods and animal feeds, final guidance[EB/OL].https://www.fda.gov/regulatory-information/search-fda-guidance-documents/guidance-industry-fumonisin-levels-human-foods-and-animal-feeds.
    The codex committee on food additives and contaminants. general standard for contaminants and toxins in food and feed : CODEX STAN 193-1995 [S]. Washington DC: FAO, 1995.
    The Commission of the European Communities. setting maximum levels for certain contaminants in food stuffs : EC No 1881/2006 [S]. Brussel: official journal of the European Union, 2006.
    国家质量监督检验检疫总局, 中国国家标准化管理委员会. 饲料卫生标准: GB 13078-2017[S]. 北京: 中国标准出版社, 2017.
    谢刚, 王松雪. 粮油真菌毒素检测技术及标准物质研究进展[C]. 北京: 国家真菌毒素防控科技创新联盟成立大会暨第一届中国真菌毒素大会论文集, 2016.
    韩铮, 郭文博, 范楷, 等. 真菌毒素检测及相关标准物质制备技术研究 [C]. 北京: 国家真菌毒素防控科技创新联盟成立大会暨第一届中国真菌毒素大会论文集, 2016.
    卢晓华, 薄梦, 吴雪, 等. 标准物质领域发展现状及趋势[J]. 化学试剂, 2022, 44(10): 1403-1410.
    GUO Z, LI X, LI H. Certified reference materials and metrological traceability for mycotoxin analysis [M]. Oxford University Press, 2019: 1695-707.
    TANGNI E K, DEBONGNIE P, HUYBRECHTS B, et al. Towards the development of innovative multi-mycotoxin reference materials as promising metrological tool for emerging and regulated mycotoxin analyses[J]. Mycotoxin Research, 2016, 33(1): 1-10.
    OLIVARES I R B, SOUZA G B, NOGUEIRA A R A, et al. Trends in developments of certified reference materials for chemical analysis-focus on food, water, soil, and sediment matrices[J]. TrAC Trends in Analytical Chemistry, 2018, 100: 53-64. doi: 10.1016/j.trac.2017.12.013
    郑子繁, 刘卫晓, 金芜军, 等. 质量平衡法及其在标准物质定值中的应用进展[J]. 生物技术进展, 2020, 10(6): 623-629.
    杨梦瑞, 简凌波, 王敏, 等. 盐酸沙拉沙星纯度定值方法研究及标准物质研制[J]. 农产品质量与安全, 2020(6): 35-44.
    张思遥, 李晓敏, 王海峰, 等. 差示扫描量热法测定4-正辛基酚, 炔雌醇等5种化合物的纯度 [J]. 计量科学与技术, 2022, (66)7: 22-27.
    韦棋, 苏福海. 甲卡西酮纯度标准物质的研制[J]. 计量科学与技术, 2020(11): 10-16.
    李硕, 张楠, 刘喆, 等. α-熊果苷纯度标准物质的研制[J]. 计量科学与技术, 2022, 66(8): 7-12.
    GUO W B, HAN Z, YANG J H, et al. Simultaneous preparation and characterization of three high-purity type B fumonisins from maize culture[J]. Analytical Methods, 2016, 8: 2737. doi: 10.1039/C5AY03307A
    国家市场监督管理总局. 标准物质的定值及均匀性、 稳定性评估: JJF 1343-2022 [S]. 北京: 中国质检出版社, 2022.
    Bezuidenhout S C, Gelderblom W C A, Gorst-Allman C P, et al. Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme[J]. Journal of the Chemical Society, Chemical Communications, 1988(11): 743-745. doi: 10.1039/c39880000743
    李莉, 李硕. QuEChERS-超高效液相色谱-串联质谱法测定玉米油中伏马毒素B1, B2, B3[J]. 食品安全质量检测学报, 2020, 11(19): 7006-7011.
    HAN Z, REN Y, LIU X, et al. A reliable isotope dilution method for simultaneous determination of fumonisins B1, B2 and B3 in traditional Chinese medicines by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry[J]. Journal of Separation Science, 2010, 33(17-18): 2723-2733. doi: 10.1002/jssc.201000423
    SZEKERES A, LORANTFY L, BENCSIK O, et al. Rapid purification method for fumonisin B1 using centrifugal partition chromatography[J]. Food Additives & Contaminants, 2013, 30(1): 147-155.
    MARIA, MÅNSSON, MARIE, et al. Isolation and NMR Characterization of Fumonisin B2 and a New Fumonisin B6from Aspergillus niger[J]. Journal of Agricultural & Food Chemistry, 2010, 58: 949-953.
    马康, 苏福海, 王海峰, 等. 有机纯度标准物质定值技术研究进展[J]. 分析测试学报, 2013, 32(7): 901-908.
    WESTWOOD S, TAICHIHUANG, TINGGARRIDO, et al. Development and validation of a suite of standards for the purity assignment of organic compounds by quantitative NMR spectroscopy[J]. Metrologia, 2019, 56: 064001. doi: 10.1088/1681-7575/ab45cb
    国家市场监督管理总局. 纯度标准物质定值计量技术规范 有机物纯度标准物质: JJF 1855-2020 [S]. 北京: 中国质检出版社, 2020.
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